Fig. 3
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12894-024-01444-5/MediaObjects/12894_2024_1444_Fig3_HTML.png)
PFK-1 suppressed the histone lactylation of BC cells and ZEB1 transcriptional activity. After sh-PFK-1 transfection and LA treatment, (A) the expression of pan-kla and H3K18la was measured by western blot. (B, C) ChIP assay showed that PFK-1 knockdown inhibited the binding of H3K18 with the promoter of ZEB1. (D, E) Luciferase assay further indicated that PFK-1 knockdown suppressed the transcription activity of ZEB1. Sh-PFK-1 transfection decreased the mRNA (F-G) and protein (H) level of ZEB1ZEB1 mRNA expression in UM-UC-1 and RT112 cell lines